Matthieu FALQUE

Meiotic recombination

     
  • plant genetics
  • molecular markers
  • linkage mapping
  • meiosis and recombination
  • modeling

 INRAE, Research Engineer

 matthieu.falque@inrae.fr —   +33 (0)1 69 33 23 64


 Atelier Cartographie, Expression et Polymorphisme
 Biologie de l'Adaptation et Systèmes en Évolution

Implication de Matthieu Falque dans axe1 70% axe1 Understanding and shedding light on evolutionary mecanisms Axe1 Implication de Matthieu Falque dans axe2 20% axe2 Understanding and predicting the genetic bases of interactions Axe2 Implication de Matthieu Falque dans axe3 10% axe3 Understanding and mobilizing cultivated diversity to provide solutions for agriculture and its actors Axe3
 Publications    

  • Génétique Quantitative et Évolution - Le Moulon
  • Université Paris-Saclay, INRAE, CNRS, AgroParisTech
  • IDEEV
  • 12 route 128
  • 91190 Gif-sur-Yvette
Matthieu FALQUE

Education and Positions

  • Currently Member of the BASE team, affiliated with the Labex Saclay Plant Sciences
  • HDR (ability to supervise PhD students) 2015, University of Orsay, France
  • Currently Head of the ACEP team (Atelier Cartographie Expression Polymorphisme)
  • Since 1998: Ingénieur de Recherche (INRA) at UMR GQE - Le Moulon, Gif-sur-Yvette, France
  • Postdoc in Plant Population Genetics (1997-1998) CNRS, Lille France
  • Postdoc in Plant Population Genetics (1995-1997) NIOO-CTO, Heteren-Wageningen, The Netherlands
  • Postdoc in Plant Molecular Genetics (1994-1995) CIRAD, Montpellier, France
  • Ph.D. in Plant Biology (1994), Univ Toulouse, France

Research interests

My research in the BASE team

2 pathways for crossover formation

2 pathways for crossover formation

Starting from linkage mapping for plant breeding applications, my research now mostly focuses on crossover number and distribution. In particular, I have been developing with Olivier Martin mathematical models to simulate crossover interference. These models take into account the two different pathways of crossover formation which have been discovered in several organisms. Using these modeling approaches, we have unveiled the existence of these two distinct pathways of crossover formation in Maize, and investigated quantitative properties of crossover formation mechanisms in different species. In particular, we have demonstrated in Tomato that crossovers produced by two different pathways still can interfere with each other out to distances of about 6 microns.

Fluorescent markers FACS-based method for high-throughput measurement of recombination rate and crossover interference in the budding yeast

FACS recombination phenotyping (simple case of 2 markers)

More recently, with my PhD student Xavier Raffoux , we have developed experimental approaches using the budding yeast as a model species to investigate the genetics underlying quantitative variability of recombination rate and landscape along chromosomes.
First, we have developed a method to measure recombination rate and crossover interference at high-throughput by using fluorescent markers. We have developed 8 tester strains spanning entirely chromosomes VI and XI, and parly chromosome I. In each tester, 3 linked fluorescent markers have been inserted at about 30cM from each other, thus delimiting 2 consecutive intervals from which crossover interference can be measured with a very high accurracy (Ref).
Then we have applied this method to characterize the intra-specific diversity of recombination rate and interference in the species S. cerevisiae. In the different intervals analyzed, recombination showed up to 9-fold variation across strains but global recombination landscapes along chromosomes varied less. We also measured recombination rates in one region in 10 different crosses involving five parental strains. Our overall results indicate that recombination rate is increasingly positively correlated with sequence similarity between homologs. We also estimated that cis and trans effects explained respectively 38% and 17% of the variance of recombination rate(Ref).
Our main research interest now is about evolutionary aspects of meiotic recombination. Using our FACS-based method, we will implement experimental evolution under recurrent selection for modified increased or dereased rate, and use the evolved populations to investigate the role of recombination in adaptation to stress.

My research in the ACEP group

The ACEP group is an internal platform of services in the fields of genetic map construction and analyses of DNA polymorphism and gene expression. We provide DNA analysis service and keep a technological witch in molecular marker technologies. I also develop software tools for automated genetic mapping with large numbers of markers, and I have constructed high-density genetic maps in several plant species.

Current Projects

  1. Experimental evolution under recurrent selection for meiotic recombination rate
  2. Automatic construction of high-resolution linkage maps from data sets with high numbers of markers.
  3. Quantifying across successive generations how linkage slows down genetic progress and how to best use new technologies that enhance recombination rates.

Teaching activities

Occasional teaching for students and professionals from breeding companies on molecular markers, genetic mapping, and plant genomics for breeding.

Publications

  • Boideau F., Huteau V., Maillet L., Brunet A., Coriton O., Deniot G., Trotoux G., Taburel-Lodé M., Eber F., Gilet M., Baron C., Boutte J., Richard G., Aury JM., Belser C., Labadie K., Morice J., Falentin C., Martin O., Falque M. , Chèvre AM., Rousseau-Gueutin M.. (2024) Alternating between even and odd ploidy levels switches on and off the recombination control, even near the centromeres. The Plant Cell, koae208
  • Falque M. , Bourgais A., Dumas F., De Carvalho M., Diblasi C.. (2024) MiniRead: A simple and inexpensive do‐it‐yourself device for multiple analyses of micro‐organism growth kinetics. Yeast, yea.3932
  • Bina H., Yousefzadeh H., Venon A., Remoué C., Rousselet A., Falque M. , Faramarzi S., Chen X., Samanchina J., Gill D., Kabaeva A., Giraud T., Hosseinpour B., Abdollahi H., Gabrielyan I., Nersesyan A., Cornille A.. (2022) Evidence of an additional centre of apple domestication in Iran, with contributions from the Caucasian crab apple Malus orientalis Uglitzk. to the cultivated apple gene pool. Molecular Ecology, 21 (31) 5581-5601
  • Hsu YM., 2022-09, Mining genetic diversity for tomorrow's agriculture, Theses, Université Paris-Saclay
  • Hsu YM., Falque M. , Martin OC.. (2022) Quantitative modelling of fine-scale variations in the Arabidopsis thaliana crossover landscape. Quant Plant Bio., (3) e3
  • Vidal A., Gauthier F., Rodrigez W., Guiglielmoni N., Leroux D., Chevrolier N., Jasson S., Tourrette E., Martin OC., Falque M. . (2022) SeSAM: software for automatic construction of order-robust linkage maps. BMC Bioinformatics, 1 (23) 499
  • Olvera-Vazquez SG., Alhmedi A., Miñarro M., Shykoff JA., Marchadier E., Rousselet A., Remoué C., Gardet R., Degrave A., Robert P., Chen X., Porchier J., Giraud T., Vander-Mijnsbrugee K., Raffoux X., Falque M. , Alins G., Didelot F., Beliën T., Dapena E., Lemarquand A., Cornille A.. (2021) Experimental test for local adaptation of the rosy apple aphid (Dysaphis plantaginea) to its host (Malus domestica) and to its climate in Europe. PCI Ecology, (Pre-registration version)
  • Olvera-Vazquez SG., Remoué C., Venon A., Rousselet A., Grandcolas O., Azrine M., Momont L., Galan M., Benoit L., David GM., Alhmedi A., Beliën T., Alins G., Franck P., Haddioui A., Jacobsen SK., Andreev R., Simon S., Sigsgaard L., Guibert E., Tournant L., Gazel F., Mody K., Khachtib Y., Roman A., Ursu TM., Zakharov IA., Belcram H., Harry M., Roth M., Simon JC., Oram S., Ricard JM., Agnello A., Beers EH., Engelman J., Balti I., Salhi-Hannachi A., Zhang H., Tu H., Mottet C., Barrès B., Degrave A., Razmjou J., Giraud T., Falque M. , Dapena E., Miñarro M., Jardillier L., Deschamps P., Jousselin E., Cornille A.. (2021) Large-scale geography survey provides insights into the colonization history of a major aphid pest on its cultivated apple host in Europe, North America and North Africa. Peer Community Journal, (1)
  • Tourrette E., Falque M. , Martin OC.. (2021) Enhancing backcross programs through increased recombination. Genet Sel Evol, 1 (53) 25
  • Carrillo-Perdomo E., Vidal A., Kreplak J., Duborjal H., Leveugle M., Duarte J., Desmetz C., Deulvot C., Raffiot B., Marget P., Tayeh N., Pichon JP., Falque M. , Martin OC., Burstin J., Aubert G.. (2020) Development of new genetic resources for faba bean (Vicia faba L.) breeding through the discovery of gene-based SNP markers and the construction of a high-density consensus map. Sci Rep, 1 (10) 6790
  • Falque M. , Jebreen K., Paux E., Knaak C., Mezmouk S., Martin OC.. (2020) CNVmap: A Method and Software To Detect and Map Copy Number Variants from Segregation Data. Genetics, 3 (214) 561-576
  • Courret C., Gérard PR., Ogereau D., Falque M. , Moreau L., Montchamp-Moreau C.. (2019) X-chromosome meiotic drive in Drosophila simulans: a QTL approach reveals the complex polygenic determinism of Paris drive suppression. Heredity, 6 (122) 906-915
  • Fustier MA., Martínez-Ainsworth NE., Aguirre-Liguori JA., Venon A., Corti H., Rousselet A., Dumas F., Dittberner H., Camarena MG., Grimanelli D., Ovaskainen O., Falque M. , Moreau L., Meaux J., Montes-Hernández S., Eguiarte LE., Vigouroux Y., Manicacci D., Tenaillon MI.. (2019) Common gardens in teosintes reveal the establishment of a syndrome of adaptation to altitude. PLOS Genetics, 12 (15) e1008512
  • Kreplak J., Madoui MA., Cápal P., Novák P., Labadie K., Aubert G., Bayer PE., Gali KK., Syme RA., Main D., Klein A., Bérard A., Vrbová I., Fournier C., d’Agata L., Belser C., Berrabah W., Toegelová H., Milec Z., Vrána J., Lee HT., Kougbeadjo A., Térézol M., Huneau C., Turo CJ., Mohellibi N., Neumann P., Falque M. , Gallardo K., McGee R., Tar’an B., Bendahmane A., Aury JM., Batley J., Le Paslier MC., Ellis N., Warkentin TD., Coyne CJ., Salse J., Edwards D., Lichtenzveig J., Macas J., Doležel J., Wincker P., Burstin J.. (2019) A reference genome for pea provides insight into legume genome evolution. Nat Genet, 9 (51) 1411-1422
  • Martinez Palacios P., Jacquemot MP., Tapie M., Rousselet A., Diop M., Remoué C., Falque M. , Lloyd A., Jenczewski E., Lassalle G., Chévre AM., Lelandais C., Crespi M., Brabant P., Joets J., Alix K.. (2019) Assessing the Response of Small RNA Populations to Allopolyploidy Using Resynthesized Brassica napus Allotetraploids. Mol Biol Evol, 4 (36) 709-726
  • Termolino P., Falque M. , Aiese Cigliano R., Cremona G., Paparo R., Ederveen A., Martin OC., Consiglio FM., Conicella C.. (2019) Recombination suppression in heterozygotes for a pericentric inversion induces the interchromosomal effect on crossovers in Arabidopsis. Plant J, 6 (100) 1163-1175
  • Tourrette E., Bernardo R., Falque M. , Martin OC.. (2019) Assessing by Modeling the Consequences of Increased Recombination in Recurrent Selection of Oryza sativa and Brassica rapa. G3, 12 (9) 4169-4181
  • Tourrette E., 25 novembre 2019, Unleashing genetic diversity in breeding by increasing recombination: an in silico study, PhD Thesis, Université de Paris-Diderot
  • Virlouvet L., El Hage F., Griveau Y., Jacquemot MP., Gineau E., Baldy A., Legay S., Horlow C., Combes V., Bauland C., Palafre C., Falque M. , Moreau L., Coursol S., Méchin V., Reymond M.. (2019) Water Deficit-Responsive QTLs for Cell Wall Degradability and Composition in Maize at Silage Stage. Front. Plant Sci., (10) 488
  • Raffoux X., 2018-11, Diversité et déterminisme génétique de la recombinaison méiotique chez Saccharomyces cerevisiae, Theses, Université Paris Saclay (COmUE)
  • Raffoux X., Bourge M., Dumas F., Martin OC., Falque M. . (2018) High-throughput measurement of recombination rates and genetic interference in Saccharomyces cerevisiae. Yeast, 6 (35) 431-442
  • Raffoux X., Bourge M., Dumas F., Martin OC., Falque M. . (2018) Role of Cis, Trans, and Inbreeding Effects on Meiotic Recombination in Saccharomyces cerevisiae. Genetics, 4 (210) 1213-1226
  • Giraud H., Bauland C., Falque M. , Madur D., Combes V., Jamin P., Monteil C., Laborde J., Palaffre C., Gaillard A., Blanchard P., Charcosset A., Moreau L.. (2017) Linkage Analysis and Association Mapping QTL Detection Models for Hybrids Between Multiparental Populations from Two Heterotic Groups: Application to Biomass Production in Maize (Zea mays L.). G3: Genes, Genomes, Genetics, g3.300121.2017
  • Giraud H., Bauland C., Falque M. , Madur D., Combes V., Jamin P., Monteil C., Laborde J., Palaffre C., Gaillard A., Blanchard P., Charcosset A., Moreau L.. (2017) Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups. Genetics, 3 (207) 1167-1180
  • Pelé A., Falque M. , Trotoux G., Eber F., Nègre S., Gilet M., Huteau V., Lodé M., Jousseaume T., Dechaumet S., Morice J., Poncet C., Coriton O., Martin OC., Rousseau-Gueutin M., Chèvre AM.. (2017) Amplifying recombination genome-wide and reshaping crossover landscapes in Brassicas. PLoS Genet, 5 (13)
  • Boutet G., Alves Carvalho S., Falque M. , Peterlongo P., Lhuillier E., Bouchez O., Lavaud C., Pilet-Nayel ML., Rivière N., Baranger A.. (2016) SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population. BMC Genomics, (17)
  • Durand E., Tenaillon MI., Raffoux X., Thépot S., Falque M. , Jamin P., Bourgais A., Ressayre A., Dillmann C.. (2015) Dearth of polymorphism associated with a sustained response to selection for flowering time in maize. BMC Evol Biol, 1 (15) 103
  • Falque M. , 2015-10-15 15/10/15, Sur les traces de la recombinaison méiotique, source de biodiversité et outil génétique, HDR, Université Paris-Sud
  • Sidhu GK., Fang C., Olson MA., Falque M. , Martin OC., Pawlowski WP.. (2015) Recombination patterns in maize reveal limits to crossover homeostasis. PNAS, 52 (112) 15982-15987
  • Tayeh N., Aluome C., Falque M. , Jacquin F., Klein A., Chauveau A., Bérard A., Houtin H., Rond C., Kreplak J., Boucherot K., Martin C., Baranger A., Pilet‐Nayel ML., Warkentin TD., Brunel D., Marget P., Paslier MCL., Aubert G., Burstin J.. (2015) Development of two major resources for pea genomics: the GenoPea 13.2K SNP Array and a high-density, high-resolution consensus genetic map. The Plant Journal, 6 (84) 1257-1273
  • Anderson LK., Lohmiller LD., Tang X., Hammond DB., Javernick L., Shearer L., Basu-Roy S., Martin OC., Falque M. . (2014) Combined fluorescent and electron microscopic imaging unveils the specific properties of two classes of meiotic crossovers. Proceedings of the National Academy of Sciences of the United States of America, 37 (111) 13415-20
  • Giraud H., Lehermeier C., Bauer E., Falque M. , Segura V., Bauland C., Camisan C., Campo L., Meyer N., Ranc N., Schipprack W., Flament P., Melchinger AE., Menz M., Moreno-Gonzalez J., Ouzunova M., Charcosset A., Schon CC., Moreau L.. (2014) Linkage disequilibrium with linkage analysis of multiline crosses reveals different multiallelic QTL for hybrid performance in the flint and dent heterotic groups of maize. Genetics, 4 (198) 1717-34
  • Jahns MT., Vezon D., Chambon A., Pereira L., Falque M. , Martin OC., Chelysheva L., Grelon M.. (2014) Crossover localisation is regulated by the neddylation posttranslational regulatory pathway. PLoS biology, 8 (12) e1001930
  • Roy S., 2014-03-31 31/03/14, Models of meiotic crossover formation, heterogeneity and inter-pathway cross-talks, PhD thesis, Université Paris Sud
  • Suay L., Zhang DS., Eber F., Jouy H., Lode M., Huteau V., Coriton O., Szadkowski E., Leflon M., Martin OC., Falque M. , Jenczewski E., Paillard S., Chevre AM.. (2014) Crossover rate between homologous chromosomes and interference are regulated by the addition of specific unpaired chromosomes in Brassica. The New phytologist, 2 (201) 645-656
  • Basu-Roy S., Gauthier F., Giraut L., Mezard C., Falque M. , Martin OC.. (2013) Hot Regions of Noninterfering Crossovers Coexist with a Nonuniformly Interfering Pathway in Arabidopsis thaliana. Genetics, 3 (195) 769-79
  • Bauer E., Falque M. , Walter H., Bauland C., Camisan C., Campo L., Meyer N., Ranc N., Rincent R., Schipprack W., Altmann T., Flament P., Melchinger AE., Menz M., Moreno-Gonzalez J., Ouzunova M., Revilla P., Charcosset A., Martin OC., Schon CC.. (2013) Intraspecific variation of recombination rate in maize. Genome biology, 9 (14) R103
  • Drouaud J., Khademian H., Giraut L., Zanni V., Bellalou S., Henderson IR., Falque M. , Mezard C.. (2013) Contrasted Patterns of Crossover and Non-crossover at Arabidopsis thaliana Meiotic Recombination Hotspots. PLoS genetics, 11 (9) e1003922
  • Sarilar V., Martinez-Palacios P., Rousselet A., Ridel C., Falque M. , Eber F., Chevre AM., Joets J., Brabant P., Alix K.. (2013) Allopolyploidy has a moderate impact on restructuring at three contrasting transposable element insertion sites in resynthesized Brassica napus allotetraploids. The New phytologist, 2 (198) 593-604
  • Ganal MW., Durstewitz G., Polley A., Bérard A., Buckler ES., Charcosset A., Clarke JD., Graner EM., Hansen M., Joets J., Le Paslier MC., McMullen MD., Montalent P., Rose M., Schön CC., Sun Q., Walter H., Martin OC., Falque M. , Lukens L.. (2011) A Large Maize (Zea mays L.) SNP Genotyping Array: Development and Germplasm Genotyping, and Genetic Mapping to Compare with the B73 Reference Genome. PLoS ONE, 12 (6) e28334
  • Gauthier F., Martin O., Falque M. . (2011) CODA (crossover distribution analyzer): quantitative characterization of crossover position patterns along chromosomes. BMC Bioinformatics, 1 (12) 27
  • Giraut L., Falque M. , Drouaud J., Pereira L., Martin OC., Mezard C.. (2011) Genome-wide crossover distribution in Arabidopsis thaliana meiosis reveals sex-specific patterns along chromosomes. PLoS genetics, 11 (7) e1002354
  • Rousset M., Bonnin I., Remoue C., Falque M. , Rhone B., Veyrieras JB., Madur D., Murigneux A., Balfourier F., Le Gouis J., Santoni S., Goldringer I.. (2011) Deciphering the genetics of flowering time by an association study on candidate genes in bread wheat (Triticum aestivum L.). Theor Appl Genet, 6 (123) 907-26
  • Virlouvet L., Jacquemot MP., Gerentes D., Corti H., Bouton S., Gilard F., Valot B., Trouverie J., Tcherkez G., Falque M. , Damerval C., Rogowsky P., Perez P., Noctor G., Zivy M., Coursol S.. (2011) The ZmASR1 protein influences branched-chain amino acid biosynthesis and maintains kernel yield in maize under water-limited conditions. Plant physiology, 2 (157) 917-36
  • Capelle V., Remoue C., Moreau L., Reyss A., Mahe A., Massonneau A., Falque M. , Charcosset A., Thevenot C., Rogowsky P., Coursol S., Prioul JL.. (2010) QTLs and candidate genes for desiccation and abscisic acid content in maize kernels. BMC plant biology, (10) 2
  • Cassan L., Moreau L., Segouin S., Bellamy A., Falque M. , Limami AM.. (2010) Genetic map construction and quantitative trait loci (QTL) mapping for nitrogen use efficiency and its relationship with productivity and quality of the biennial crop Belgian endive (Cichorium intybus L.). Journal of plant physiology, 15 (167) 1253-63
  • Storlazzi A., Gargano S., Ruprich-Robert G., Falque M. , David M., Kleckner N., Zickler D.. (2010) Recombination proteins mediate meiotic spatial chromosome organization and pairing. Cell, 1 (141) 94-106
  • Edelist C., Raffoux X., Falque M. , Dillmann C., Sicard D., Rieseberg LH., Karrenberg S.. (2009) Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae). American journal of botany, 10 (96) 1830-8
  • Falque M. , Anderson LK., Stack SM., Gauthier F., Martin OC.. (2009) Two types of meiotic crossovers coexist in maize. The Plant cell, 12 (21) 3915-25
  • Saintenac C., Falque M. , Martin OC., Paux E., Feuillet C., Sourdille P.. (2009) Detailed recombination studies along chromosome 3B provide new insights on crossover distribution in wheat (Triticum aestivum L.). Genetics, 2 (181) 393-403
  • Falque M. , Mercier R., Mézard C., de Vienne D., Martin OC.. (2007) Patterns of Recombination and MLH1 Foci Density Along Mouse Chromosomes: Modeling Effects of Interference and Obligate Chiasma. Genetics, 3 (176) 1453-1467