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Génétique Quantitative et Évolution - Le Moulon

Timothée FLUTRE

Timothée FLUTRE

Research scientist, INRAE

Quantitative genetics of wheat heterogeneous populations adapted to agroecological practices

timothee.flutre@inrae.fr

+33 (0)1 69 15 61 33

orcid.org/0000-0003-4489-4782

Publications

  • Génétique Quantitative et Évolution - Le Moulon
  • Université Paris-Saclay, INRAE, CNRS, AgroParisTech
  • Ferme du Moulon
  • F-91190 Gif-sur-Yvette

Positions and education

  • 2019-present: research scientist at UMR GQE-Le Moulon, team DEAP
  • 2014-2018: research scientist at UMR AGAP, team DAAV
  • 2011-2013: postdoctoral fellow at the University of Chicago with M. Stephens
  • 2007-2010: Ph.D. at URGI with H. Quesneville and C. Feuillet (UMR GDEC), in graduate school FIRE (ex-FDV)
  • 2003-2007: engineering school AgroParisTech; M. Sc. AIRE (ex-AIV).

Research interests

Current research

I am interested in understanding the functioning and evolution of heterogeneous populations of cultivated plants for adaptation to agro-ecological practices. I plan to conduct research on the growth dynamics and resource allocation of plants interacting with each other, with varietal mixtures of bread wheat as application. My modeling approach will depend on field experiments and farmers’ know-how, and draw on knowledge mainly from quantitative genetics, ecophysiology and statistics, but also ecology and agronomy.

Past work

Before 2019, at the INRA in Montpellier, I developed research on the genetic architecture of traits of interest for breeding in grapevine, and genomic selection in perennial fruit species in general and grapevine in particular. My approach was based on linear mixed models and high-dimensional regression for variable selection and prediction. I still have collaborations on these topics, notably in terms of multivariate statistical modeling for genome-wide association studies and genomic prediction. Before that, during my postdoc, I worked on the genetic regulation of gene transcription. To detect eQTLs jointly in multiple human tissues, I developed a multivariate hierarchical Bayesian model. During my PhD, I studied the dynamics of transposable elements (TE) and their impact on genome evolution. To annotate the repeat content of whole genomes, I developed a suite of bioinformatics tools. See selected publications on this previous topic

Selected publications

(More on ORCID and HAL)

Association genetics and genomic prediction of perennial fruit crops

  • Flutre T, Berger G, Bertrand Y, Fodor A, Launay A, Romieu C, Beccavin I, Bouckenooghe V, Roques M, Cheynier V, Bacilieri R, Boursiquot J-M, Lacombe T, Laucou V, This P, Le Cunff L, Péros J-P, Doligez A. Genome-wide association and predictionstudy of a diverse grapevine panel uncovers the genetic architecture of numerous traits of interest. In prep.
  • Launay A, Denancé C, Muranty H, Audergon J-M, Confolent C, Lambert P, Quilot-Turion B, Bert P-F, Marguerit E, Campoy J, Dirlewanger E, Quero Garcia J, Decroocq S, Decroocq V, Butterlin G, Duchêne E, Costes E, Kelner J-J, Pallas B, Mournet P, Flutre T. Combining genotyping by sequencing and genomic prediction within bi-parental crosses to speed-up the selection of perennial fruit crops. In prep.

Genetic regulation of gene expression

  • Flutre T, Wen X, Pritchard J, and Stephens M. (2013) A statistical framework for joint eQTL analysis in multiple tissues. PLoS Genetics, 9 e1003486.

Comparative genomics of transposable elements’ dynamics

  • Flutre T, Duprat E, Feuillet C, and Quesneville H. (2011) Considering transposable element diversification in de novo annotation approaches. PLoS One, 6 e16526.

PhD students

  • M. Gawinowski (2019-ongoing): co-supervision with P.-H. Cournède (CentraleSupélec); doctoral school FIRE
  • C. Brault (2018-ongoing): collaboration with her supervisors P. This, A. Doligez and L. Le Cunff (UMR AGAP); doctoral school GAIA
  • C. Bouffartigue (2016-ongoing): co-supervision with L. Hazard and N. Couix (UMR AGIR); doctoral school ABIES

Teaching

Selected software

(More on GitHub and SourceSup; many contributions to free and open-source software)

  • gbs.py: call and filter SNP genotypes from raw sequencing reads
  • demultiplex.py: demultiplex raw reads from different samples sequenced together
  • PlantBreedGame: serious game on plant breeding
  • eQtlBma: detect eQTLs jointly in multiple subgroups via Bayesian model averaging
  • REPET: detect, annotate and analyze repeats in genomic sequences, specifically designed for transposable elements.

Selected projects and funding

  • 2020-2023: member of the STABLE project coordinated by A. Gauffreteau and funded by the CASDAR

    • Assess the stability of winter wheat varieties grown in pure and in mixtures
  • 2020-2022: member of the TUHR-DRONAé project coordinated by J.-M. Gilliot and funded by AgroParisTech

    • Ultra-high-resolution remote sensing by drone for adaptation to agroecological practices
  • 2020-2021: coordinator of the PerfoMix project funded by INRAE (BAP department)

    • Performance of intra-species mixtures: explaining varietal proportions at harvest
  • 2020-2024: member of the G2WAS project coordinated by L. Torregrosa and funded by the ANR

    • Genetic architecture of the tolerance to water deficit in a perennial fruit species (V. vinifera)
  • 2016-2020: member of the SIGNE project coordinated by L. Le Cunff and funded by the CASDAR

    • Spectroscopy, a tool for the traceability and management of intra-varietal variability in grapevine
  • 2014-2018: coordinator of the genomic prediction for the EDGARR project coordinated by L. Le Cunff and funded by the CASDAR

    • Exploitation of genomic selection in order to accelerate the creation of resistant and qualitative varieties for the rosé wine industry
  • 2015-2016: coordinator of the FruitSelGen project funded by the INRA (Selgen meta-program), in addition to the SelVi project funded by the INRA (BAP department)

    • Assessment of genotyping by sequencing and genomic selection for perenial fruit crops
  • 2011-2013: post-doctoral fellowship funded by the INRA (ASC)

  • 2007-2010: PhD fellowship funded by the INRA (ASC)

Research management

  • 2020-ongoing: nominated at the scientific council of the SDV graduate school (Univ. Paris-Saclay) with D. Manicacci as substitute
  • 2015-2020: elected at the scientific council of the BAP department (INRAE) as substitute of J. Ronfort (UMR AGAP)
  • 2014-2019: elected at the council of the UMR AGAP

Publications

Bouffartigue C, Debille S, Fabreguettes O, Cabrer AR, Pereira-Lorenzo S, Flutre T, Harvengt L. (2020) Two main genetic clusters with high admixture between forest and cultivated chestnut (Castanea sativa Mill.) in France. Annals of Forest Science, 3 (77) 74
Tello J, Torres-Pérez R, Flutre T, Grimplet J, Ibáñez J. (2020) VviUCC1 Nucleotide Diversity, Linkage Disequilibrium and Association with Rachis Architecture Traits in Grapevine. Genes, 6 (11) 598
Flutre T, Bacilieri R, Bécavin I, Berger G, Bertrand Y, Boursiquot JM, Fodor A, Lacombe T, Laucou V, Launay A, Le Cunff L, Romieu C, This P, Péros JP, Doligez A. (2019) Genome-wide association study of a diverse grapevine panel: example of berry weight. Acta Hortic., 1248 227-234
Flutre T, Diot J, David J. (2019) PlantBreedGame: A Serious Game that Puts Students in the Breeder’s Seat. Crop Science, 0 (52) 1-2
Tello J, Roux C, Chouiki H, Laucou V, Sarah G, Weber A, Santoni S, Flutre T, Pons T, This P, Péros JP, Doligez A. (2019) A novel high-density grapevine (Vitis vinifera L.) integrated linkage map using GBS in a half-diallel population. Theor Appl Genet, 8 (132) 2237-2252
Moisy C, Berger G, Flutre T, Le Cunff L, Péros JP. (2017) Quantitative assessment of grapevine wood colonization by the dieback fungus Eutypa lata. Journal of Fungi, 2 (3) 21
The GTEx Consortium, Ardlie K, Deluca D, Segrè A, Sullivan T, Young T, Gelfand E, Trowbridge C, Maller J, Tukiainen T, Lek M, Ward L, Kheradpour P, Iriarte B, Meng Y, Palmer C, Esko T, Winckler W, Hirschhorn J, Kellis M, MacArthur D, Getz G, Shabalin A, Li G, Zhou YH, Nobel A, Rusyn I, Wright F, Lappalainen T, Ferreira P, Ongen H, Rivas M, Battle A, Mostafavi S, Monlong J, Sammeth M, Mele M, Reverter F, Goldmann J, Koller D, Guigó R, McCarthy M, Dermitzakis E, Gamazon E, Im H, Konkashbaev A, Nicolae D, Cox N, Flutre T, Wen X, Stephens M, Pritchard J, Tu Z, Zhang B, Huang T, Long Q, Lin L, Yang J, Zhu J, Liu J, Brown A, Mestichelli B, Tidwell D, Lo E, Salvatore M, Shad S, Thomas J, Lonsdale J, Moser M, Gillard B, Karasik E, Ramsey K, Choi C, Foster B, Syron J, Fleming J, Magazine H, Hasz R, Walters G, Bridge J, Miklos M, Sullivan S, Barker L, Traino H, Mosavel M, Siminoff L, Valley D, Rohrer D, Jewell S, Branton P, Sobin L, Barcus M, Qi L, McLean J, Hariharan P, Um K, Wu S, Tabor D, Shive C, Smith A, Buia S, Undale A, Robinson K, Roche N, Valentino K, Britton A, Burges R, Bradbury D, Hambright K, Seleski J, Korzeniewski G, Erickson K, Marcus Y, Tejada J, Taherian M, Lu C, Basile M, Mash D, Volpi S, Struewing J, Temple G, Boyer J, Colantuoni D, Little R, Koester S, Carithers L, Moore H, Guan P, Compton C, Sawyer S, Demchok J, Vaught J, Rabiner C, Lockhart N, Ardlie K, Getz G, Wright F, Kellis M, Volpi S, Dermitzakis E. (2015) Human genomics. The Genotype-Tissue Expression (GTEx) pilot analysis: multitissue gene regulation in humans.. Science, 6235 (348) 648-660
Andújar B, Campderrós G, García M, Marino M, Mas M, Narbona L, Pérez L, Rodrigo P, Velásquez A, Vilà J, Laguna D, Flutre T, Bonnefond M, Sescousse G, Riboli-Sasco L. (2015) Twenty Tips for High-School Students Engaging in Research with Scientists. Frontiers for Young Minds, (3)
Fagny M, Patin E, MacIsaac JL, Rotival M, Flutre T, Jones MJ, Siddle KJ, Quach H, Harmant C, McEwen LM, Froment A, Heyer E, Gessain A, Betsem E, Mouguiama-Daouda P, Hombert JM, Perry GH, Barreiro LB, Kobor MS, Quintana-Murci L. (2015) The epigenomic landscape of African rainforest hunter-gatherers and farmers. Nature Communications, (6)
Flutre T, Wen X, Pritchard J, Stephens M. (2013) A statistical framework for joint eQTL analysis in multiple tissues. PLoS Genetics, 5 (9) e1003486
Bigot Y, Permal E, Flutre T, Quesneville H. (2012) Roadmap for annotating transposable elements in eukaryote genomes. , (859) 53-68
Flutre T, Permal E, Quesneville H, Grandbastien MA, Casacuberta J. (2012) Transposable element annotation in completely sequenced eukaryote genomes. , (24) 17-39
Leroy P, Guilhot N, Sakai H, Bernard A, Choulet F, Theil S, Reboux S, Amano N, Flutre T, Pelegrin C, Ohyanagi H, Seidel M, Giacomoni F, Reichstadt M, Alaux M, Gicquello E, Legeai F, Cerutti L, Numa H, Tanaka T, Mayer K, Itoh T, Quesneville H, Feuillet C. (2012) TriAnnot: A Versatile and High Performance Pipeline for the Automated Annotation of Plant Genomes.. Frontiers in Plant Science, (3)
Ségurel L, Thompson E, Flutre T, Lovstad J, Venkat A, Margulis S, Moyse J, Ross S, Gamble K, Sella G, Ober C, Przeworski M. (2012) The ABO blood group is a trans-species polymorphism in primates. Proceedings of the National Academy of Sciences, 45 (109) 18493-18498
Flutre T, Julou T, Riboli-Sasco L, Ribrault C. (2011) Pilot scheme for misconduct database. Nature, 37 (478) 37-37
Flutre T, Duprat E, Feuillet C, Quesneville H. (2011) Considering transposable element diversification in de novo annotation approaches. PLoS One, 1 (6) e16526
Flutre T, Permal E, Quesneville H. (2011) In search of lost trajectories: recovering the diversification of transposable elements. Mobile Genetic Elements, 2 (1)